Part:BBa_K4432142:Design

Pseudomonas aeruginosa PhzA1-G1 truncated operon under the control of the T5 promoter

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1048
  Illegal EcoRI site found at 3670
  Illegal PstI site found at 1191
  Illegal PstI site found at 4675
  Illegal PstI site found at 5224
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1048
  Illegal EcoRI site found at 3670
  Illegal PstI site found at 1191
  Illegal PstI site found at 4675
  Illegal PstI site found at 5224
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1048
  Illegal EcoRI site found at 3670
  Illegal BamHI site found at 397
  Illegal BamHI site found at 1603
  Illegal XhoI site found at 341
  Illegal XhoI site found at 500
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1048
  Illegal EcoRI site found at 3670
  Illegal PstI site found at 1191
  Illegal PstI site found at 4675
  Illegal PstI site found at 5224
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1048
  Illegal EcoRI site found at 3670
  Illegal PstI site found at 1191
  Illegal PstI site found at 4675
  Illegal PstI site found at 5224
  Illegal NgoMIV site found at 263
  Illegal NgoMIV site found at 1272
  Illegal NgoMIV site found at 1419
  Illegal NgoMIV site found at 1437
  Illegal NgoMIV site found at 1441
  Illegal NgoMIV site found at 2245
  Illegal NgoMIV site found at 2419
  Illegal AgeI site found at 5513
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 5656
  Illegal BsaI.rc site found at 2355
  Illegal BsaI.rc site found at 4732
  Illegal BsaI.rc site found at 5138
  Illegal BsaI.rc site found at 5165
  Illegal SapI.rc site found at 4152
  

Design Notes

T5 promoter (BBa_K4432000) + custom made RBS (BBa_K4432014) + Pseudomonas aeruginosa PhzA1-G1 truncated operon (BBa_I723028)

Source

PCR amplification & Assembly